Proteintech, 22102-1-AP, POMC Polyclonal antibody

Size: 20ul / 150ul The POMC (22102-1-AP) by Proteintech is a Polyclonal antibody targeting POMC in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 22102-1-AP targets POMC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse pituitary gland tissue, Transfected HEK-293 cells Positive IHC detected in: human pituitary tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes, including regulating energy metabolism, immune reactions, and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP, γ-MSH, ACTH, α-MSH, CLIP, Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin. Specification Tested Reactivity: human, mouse Cited Reactivity: rat, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag17311 Product name: Recombinant human POMC protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 27-176 aa of BC065832 Sequence: WCLESSQCQDLTTESNLLECIRACKPDLSAETPMFPGNGDEQPLTENPRKYVMGHFRWDRFGRRNSSSSGSSGAGQKREDVSAGEDCGPLPEGGPEPRSDGAKPGPREGKRSYSMEHFRWGKPVGKKRRPVKVYPNGAEDESAEAFPLEF Predict reactive species Full Name: proopiomelanocortin Calculated Molecular Weight: 267 aa, 29 kDa Observed Molecular Weight: 35 kDa GenBank Accession Number: BC065832 Gene Symbol: POMC Gene ID (NCBI): 5443 RRID: AB_3085685 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P01189 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.