Proteintech, 22246-1-AP, TBP Polyclonal antibody

Size: 20ul / 150ul The TBP (22246-1-AP) by Proteintech is a Polyclonal antibody targeting TBP in WB, IF/ICC, ELISA applications with reactivity to human, mouse samples 22246-1-AP targets TBP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Jurkat cells, COLO 320 cells, HeLa cells, mouse colon tissue, SKOV-3 cells Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information TBP, TATA box-binding protein, is a DNA-binding subunit of TFIID, which is a multisubunit complex essential for the expression of most protein-encoding genes. The well-conserved C-terminal domains of 180 aa is both necessary and sufficient for interaction with DNA and for assembly of the basal transcription apparatus.The N terminus of TBP modulates the DNA-binding activity of the C terminus of the protein. Once binds to the TATA box mediated by TBP, TFIID initiate the transcription step of the pre-initiation complex(PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. This is a rabbit polyclonal antibody raised against part of N-terminal chain of human TBP, and specially recognize a ~46 kDa band. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag17740 Product name: Recombinant human TBP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-140 aa of BC110341 Sequence: MDQNNSLPPYAQGLASPQGAMTPGIPIFSPMMPYGTGLTPQPIQNTNSLSILEEQQRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQAVAAAAVQQSTSQQATQGTSGQAPQLFHSQTLTTAPLPGTTPLYPS Predict reactive species Full Name: TATA box binding protein Calculated Molecular Weight: 338 aa, 38 kDa Observed Molecular Weight: mouse/rat 33-36 kDa and human 37-43kDa GenBank Accession Number: BC110341 Gene Symbol: TBP Gene ID (NCBI): 6908 RRID: AB_2879046 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P20226 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.