Proteintech, 22283-1-AP, CLTCL1 Polyclonal antibody

Size: 20ul / 150ul The CLTCL1 (22283-1-AP) by Proteintech is a Polyclonal antibody targeting CLTCL1 in IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 22283-1-AP targets CLTCL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive IP detected in: HeLa cells Positive IHC detected in: mouse testis tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CLTCL1, also called , CLTCL, and CLTD, belongs to the clathrin heavy chain family. Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. The antibody is specific to CLTCL1. It has no cross-reaction to CLTC. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: clathrin, heavy chain-like 1 Calculated Molecular Weight: 187 kDa Observed Molecular Weight: 180-210 kDa GenBank Accession Number: NM_007098 Gene Symbol: CLTCL1 Gene ID (NCBI): 8218 RRID: AB_11183764 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P53675 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.