Proteintech, 22302-1-AP, COL6A6 Polyclonal antibody

Size: 20ul / 150ul The COL6A6 (22302-1-AP) by Proteintech is a Polyclonal antibody targeting COL6A6 in WB, IHC, ELISA applications with reactivity to human, Mouse samples 22302-1-AP targets COL6A6 in WB, IHC, ELISA applications and shows reactivity with human, Mouse samples. Tested Applications Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue Positive IHC detected in: mouse heart tissue, mouse lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Collagen type VI alpha 6 chain (COL6A6) belongs to the collagen VI (COL6) family which acts as a vital role in the extracellular matrix. COL6A6 encodes a 2,262-amino acid protein that contains multiple von Willebrand factor domains and forms a component of the basal lamina of epithelial cells (PMID: 33747955). COL6A6 is a novel tumor suppressor gene in NSCLC and can serve as a potentially valuable prognostic biomarker and therapeutic target for NSCLC (PMID: 35116307). Specification Tested Reactivity: human, Mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: collagen type VI alpha 6 Calculated Molecular Weight: 247 kDa Observed Molecular Weight: 167 kDa, 250 kDa GenBank Accession Number: NM_001102608 Gene Symbol: COL6A6 Gene ID (NCBI): 131873 RRID: AB_3085695 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: A6NMZ7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.