Proteintech, 22899-1-AP, LMTK3 Polyclonal antibody

Size: 20ul / 150ul The LMTK3 (22899-1-AP) by Proteintech is a Polyclonal antibody targeting LMTK3 in WB, IHC, ELISA applications with reactivity to human samples 22899-1-AP targets LMTK3 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MCF-7 cells Positive IHC detected in: human breast cancer tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information LMTK3 is enriched at a perinuclear structure around Golgi membranes, it is present as a dotted pattern throughout neurites, and localized at neuronal synapses. It is also expected to be expressed in brain and duodenum. LMTK3 is a serine/threonine protein kinase expressed predominantly in brain. LMTK3 regulated ER-alpha stability and activity directly at the mRNA level via downregulation of PKC catalytic activity, resulting in less phosphorylated AKT at ser473. In vitro kinase assays indicated that LMTK3 inhibited the ability of PKC to phosphorylate ER-alpha, thereby affecting ER-alpha activity indirectly at the protein level by protecting it from proteasomal degradation. In agreement, analysis with a breast tumor xenograft mouse model revealed that knockdown of LMTK3 reduced proliferation of ER-alpha-positive breast cancer cells and reduced tumor growth. In addition, LMTK3 abundance and intronic polymorphisms were significantly associated with human cancers, overall patient survival, and predicted response to endocrine therapies. The calculated molecular weight of LMTK3 is 153 kDa. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: lemur tyrosine kinase 3 Calculated Molecular Weight: 154 kDa Observed Molecular Weight: ~155 kDa GenBank Accession Number: NM_001080434 Gene Symbol: LMTK3 Gene ID (NCBI): 114783 RRID: AB_3085704 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen Affinity purified UNIPROT ID: Q96Q04 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.