Proteintech, 23404-1-AP, WDR62 Polyclonal antibody

Size: 20ul / 150ul The WDR62 (23404-1-AP) by Proteintech is a Polyclonal antibody targeting WDR62 in IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 23404-1-AP targets WDR62 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive IP detected in: HEK-293T cells Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: hTERT-RPE1 cells, A549 cells Recommended dilution Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information The WD40-repeat protein 62 (WDR62) is a scaffold protein that recruits different components of the JNK signaling pathway to regulate several human diseases including neurological disorders, infertility, and tumorigenesis (PMID: 33937237). Moreover, WDR62 participates in diverse biological processes, especially mitotic spindle organization via regulating centriole biogenesis and the function of centriole-associated protein (PMID: 35093571). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag19865 Product name: Recombinant human WDR62 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 281-630 aa of BC017261 Sequence: CLTSLASCVPASSVLPTDRNLPTPTSAPTPGLAQGVHAPSTCSYMEATASSRARISRSISLGDSEGPIVATLAQPLRRPSSVGELASLGQELQAITTATTPSLDSEGQEPALRSWGNHEARANLRLTLSSACDGLLLPPVDTQPGVTVPAVSFPAPSPVEESALRLHGSAFRPSLPAPESPGLPAHPSNPQLPEARPGIPGSTASLLEPTSGALGLFQGSPARWSEPWVPVEALPPSPLELSRVGNILHRLQTTFQEALDLYRVLVSSGQVDTGQQQARTELVSTFLWIHSQLEAECLVGTSVAPAQALPSPGPPSPPTLYPLASPDLQALLEHYSELLVQAVRRKARGH Predict reactive species Full Name: WD repeat domain 62 Calculated Molecular Weight: 1518 aa, 166 kDa Observed Molecular Weight: 180 kDa GenBank Accession Number: BC017261 Gene Symbol: WDR62 Gene ID (NCBI): 284403 RRID: AB_3085711 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen Affinity purified UNIPROT ID: O43379 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.