Proteintech, 23599-1-AP, RPS25 Polyclonal antibody

Size: 20ul / 150ul The RPS25 (23599-1-AP) by Proteintech is a Polyclonal antibody targeting RPS25 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 23599-1-AP targets RPS25 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, HeLa cells, K-562 cells, MCF-7 cells Positive IHC detected in: human breast cancer tissue, human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information Ribosomal protein S25 (RPS25) is a novel MDM2 interacting protein. It may be involved in viral replication of Dicistroviridae and hepatitis C viruses. RPS25 is overexpressed in human leukemia cells exhibiting adriamycin resistance. It may have a role in p53-mediated apoptosis and cell-cycle arrest. The expected MW of RPS25 is about 15-17kDa. Catalog# 23599-1-AP is a rabbit polyclonal antibody raised against full-length RPS25, and is recommended for ELISA, Western blotting. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7890 Product name: Recombinant human RPS25 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-125 aa of BC004986 Sequence: MPPKDDKKKKDAGKSAKKDKDPVNKSGGKAKKKKWSKGKVRDKLNNLVLFDKATYDKLCKEVPNYKLITPAVVSERLKIRGSLARAALQELLSKGLIKLVSKHRAQVIYTRNTKGGDAPAAGEDA Predict reactive species Full Name: ribosomal protein S25 Calculated Molecular Weight: 14 kDa Observed Molecular Weight: 15-17 kDa GenBank Accession Number: BC004986 Gene Symbol: RPS25 Gene ID (NCBI): 6230 RRID: AB_11183757 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P62851 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.