Proteintech, 23925-1-AP, SOX7 Polyclonal antibody

Size: 20ul / 150ul The SOX7 (23925-1-AP) by Proteintech is a Polyclonal antibody targeting SOX7 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 23925-1-AP targets SOX7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: COLO 320 cells, mouse heart tissue, PC-3 cells Positive IHC detected in: human prostate cancer tissue, human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information SOX7, also named as Transcription factor SOX-7, is a 388 amino acid protein, which is widely expressed in adult and fetal tissues and tends to be down-regulated in prostate adenocarcinomas and colorectal tumors due to promoter hypermethylation. SOX7 binds to and activates the CDH5 promoter, hence plays a role in the transcriptional regulation of genes expressed in the hemogenic endothelium and blocks further differentiation into blood precursors. SOX7 may be required for the survival of both hematopoietic and endothelial precursors during specification. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10323 Product name: Recombinant human SOX7 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-388 aa of BC071947 Sequence: MASLLGAYPWPEGLECPALDAELSDGQSPPAVPRPPGDKGSESRIRRPMNAFMVWAKDERKRLAVQNPDLHNAELSKMLGKSWKALTLSQKRPYVDEAERLRLQHMQDYPNYKYRPRRKKQAKRLCKRVDPGFLLSSLSRDQNALPEKRSGSRGALGEKEDRGEYSPGTALPSLRGCYHEGPAGGGGGGTPSSVDTYPYGLPTPPEMSPLDVLEPEQTFFSSPCQEEHGHPRRIPHLPGHPYSPEYAPSPLHCSHPLGSLALGQSPGVSMMSPVPGCPPSPAYYSPATYHPLHSNLQAHLGQLSPPPEHPGFDALDQLSQVELLGDMDRNEFDQYLNTPGHPDSATGAMALSGHVPVSQVTPTGPTETSLISVLADATATYYNSYSVS Predict reactive species Full Name: SRY (sex determining region Y)-box 7 Calculated Molecular Weight: 388 aa, 42 kDa Observed Molecular Weight: 42-45 kDa GenBank Accession Number: BC071947 Gene Symbol: SOX7 Gene ID (NCBI): 83595 RRID: AB_2879363 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BT81 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.