Proteintech, 24033-1-AP, ANKRD57 Polyclonal antibody

Size: 20ul / 150ul The ANKRD57 (24033-1-AP) by Proteintech is a Polyclonal antibody targeting ANKRD57 in WB, IP, IHC, ELISA applications with reactivity to human samples 24033-1-AP targets ANKRD57 in WB, IP, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A431 cells, HeLa cells, HepG2 cells Positive IP detected in: HeLa cells Positive IHC detected in: human cerebellum tissue, human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:20000-1:100000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag21238 Product name: Recombinant human ANKRD57 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-306 aa of BC036652 Sequence: MEGPAEWGPEAALGPEAVLRFLAERGGRALHAELVQHFRGALGGEPEQRARARAHFKELVNAVATVRVDPADGAKYVHLKKRFCEGPSEPSGDPPRIQVTAEPEAPDGPAGPEARDRLPDAAAPESLPGQGRELGEGEPPAPAHWPPLSAGARRKNSRRDVQPLPRTPAPGPSEDLELPPHGCEEADRGSSLVGATAQRPARQNLRDLVMGSSPQLKRSVCPGGSSPGSSSGGGRGRGGGDSDSASVASSSAEEESSGGGSVTLDPLEHAWMLSASDGKWDSLEGLLTCEPGLLVKRDFITGFTCL Predict reactive species Full Name: ankyrin repeat domain 57 Calculated Molecular Weight: 525 aa, 56 kDa Observed Molecular Weight: 65-70 kDa GenBank Accession Number: BC036652 Gene Symbol: ANKRD57 Gene ID (NCBI): 65124 RRID: AB_2879412 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen Affinity purified UNIPROT ID: Q53LP3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.