Proteintech, 24049-1-AP, Cytokeratin 23 Polyclonal antibody

Size: 20ul / 150ul The Cytokeratin 23 (24049-1-AP) by Proteintech is a Polyclonal antibody targeting Cytokeratin 23 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 24049-1-AP targets Cytokeratin 23 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: BxPC-3 cells, HepG2 cells, HeLa cells Positive IHC detected in: human colon cancer tissue, human skin cancer tissue, human placenta tissue, human liver cancer tissue, rat colon tissue, mouse colon tissue, human bowen diseaseNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT23 belongs to type I keratins. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag21129 Product name: Recombinant human KRT23 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1-422 aa of BC028356 Sequence: MNSGHSFSQTPSASFHGAGGGWGRPRSFPRAPTVHGGAGGARISLSFTTRSCPPPGGSWGSGRSSPLLGGNGKATMQNLNDRLASYVEKVRALEEANMKLESRILKWHQQRDPGSKKDYSQYEENITHLQEQIVDGKMTNAQIILLIDNARMAVDDFNLKYENEHSFKKDLEIEVEGLRRTLDNLTIVTTDLEQEVEGMRKELILMKKHHEQEMEKHHVPSDFNVNVKVDTGPREDLIKVLEDMRQEYELIIKKKHRDLDTWYKEQSAAMSQEAASPATVQSRQGDIHELKRTFQALEIDLQTQYSTKSALENMLSETQSRYSCKLQDMQEIISHYEEELTQLRHELERQNNEYQVLLGIKTHLEKEITTYRRLLEGESEGTREESKSSMKVFATPKIKAITQETINGRLVLCQVNEIQKHA Predict reactive species Full Name: keratin 23 (histone deacetylase inducible) Calculated Molecular Weight: 422 aa, 48 kDa Observed Molecular Weight: 48 kDa GenBank Accession Number: BC028356 Gene Symbol: KRT23 Gene ID (NCBI): 25984 RRID: AB_2879416 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9C075 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.