Proteintech, 24064-1-AP, Matrilin 2 Polyclonal antibody

Size: 20ul / 150ul The Matrilin 2 (24064-1-AP) by Proteintech is a Polyclonal antibody targeting Matrilin 2 in WB, IHC, ELISA applications with reactivity to human, mouse samples 24064-1-AP targets Matrilin 2 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: NIH/3T3 cells, HEK-293 cells, mouse liver tissue Positive IHC detected in: mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information The matrilins represent a new family of oligomeric non-collagenous extracellular matrix proteins that are assumed to act as adapter molecules connecting other proteins and proteoglycans in the extracellular matrix (PMID: 17513098). Four matrilins (matrilin-1, -2, -3, and -4) have been identified. They all share a structure made up of von Willebrand factor A domains, EGF-like domains and a coiled-coil alpha-helical module (PMID: 18386166). Matrilin-1 and matrilin-3 are expressed mainly in cartilage, while matrilin-2 and matrilin-4 show broader tissue distribution. Matrilin-2 is the largest member of the family. It incorporates into loose and dense connective tissue and becomes associated with some basement membranes (PMID: 17513098). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag21113 Product name: Recombinant human MATN2 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 26-351 aa of BC010444 Sequence: RSRGRSISRGRHARTHPQTALLESSCENKRADLVFIIDSSRSVNTHDYAKVKEFIVDILQFLDIGPDVTRVGLLQYGSTVKNEFSLKTFKRKSEVERAVKRMRHLSTGTMTGLAIQYALNIAFSEAEGARPLRENVPRVIMIVTDGRPQDSVAEVAAKARDTGILIFAIGVGQVDFNTLKSIGSEPHEDHVFLVANFSQIETLTSVFQKKLCTAHMCSTLEHNCAHFCINIPGSYVCRCKQGYILNSDQTTCRIQDLCAMEDHNCEQLCVNVPGSFVCQCYSGYALAEDGKRCVAVDYCASENHGCEHECVNADGSYLCQCHEGFA Predict reactive species Full Name: matrilin 2 Calculated Molecular Weight: 107 kDa Observed Molecular Weight: 120-150 kDa GenBank Accession Number: BC010444 Gene Symbol: Matrilin 2 Gene ID (NCBI): 4147 RRID: AB_2879422 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen Affinity purified UNIPROT ID: O00339 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.