Proteintech, 24134-1-AP, TMEM59 Polyclonal antibody

Size: 20ul / 150ul The TMEM59 (24134-1-AP) by Proteintech is a Polyclonal antibody targeting TMEM59 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 24134-1-AP targets TMEM59 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MCF-7 cells Positive IHC detected in: human breast cancer tissue, human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information TMEM59 is a regulator of autophagy in response to bacterial infection. Overexpression of this protein has been found to promote apoptosis in a glioma cell line. Alternative splicing of TMEM59 results in multiple transcript variants. Specification Tested Reactivity: human Cited Reactivity: human, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag21150 Product name: Recombinant human TMEM59 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1-324 aa of BC016374 Sequence: MAAPKGSLWVRTQLGLPPLLLLTMALAGGSGTASAEAFDSVLGDTASCHRACQLTYPLHTYPKEEELYACQRGCRLFSICQFVDDGIDLNRTKLECESACTEAYSQSDEQYACHLGCQNQLPFAELRQEQLMSLMPKMHLLFPLTLVRSFWSDMMDSAQSFITSSWTFYLQADDGKIVIFQSKPEIQYAPHLEQEPTNLRESSLSKMSSDLQMRNSQAHRNFLEDGESDGFLRCLSLNSGWILTTTLVLSVMVLLWICCATVATAVEQYVPSEKLSIYGDLEFMNEQKLNRYPASSLVVVRSKTEDHEEAGPLPTKVNLAHSEI Predict reactive species Full Name: transmembrane protein 59 Calculated Molecular Weight: 36 kDa Observed Molecular Weight: 41 kDa GenBank Accession Number: BC016374 Gene Symbol: TMEM59 Gene ID (NCBI): 9528 RRID: AB_2879439 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BXS4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.