Proteintech, 24202-1-AP, LUC7L2 Polyclonal antibody

Size: 20ul / 150ul The LUC7L2 (24202-1-AP) by Proteintech is a Polyclonal antibody targeting LUC7L2 in WB, IF/ICC, ELISA applications with reactivity to human samples 24202-1-AP targets LUC7L2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: K-562 cells, HeLa cells Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200 Background Information RNA-binding protein LUC7L2 as a negative regulator of DNA virus-triggered innate immune response. Mechanistically, LUC7L2 directly bound to intron 3 of MITA precursor messenger RNA, inhibited its splicing, and promoted its nonsense-mediated decay, leading to its downregulation at the protein level (PMID:34155193). Moreover, LUC7L2 as a component of the U1 snRNP capable of reprogramming cellular metabolism through changes in alternative pre-mRNA splicing (PMID:33961773). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag21135 Product name: Recombinant human LUC7L2 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1-392 aa of BC017163 Sequence: MSAQAQMRAMLDQLMGTSRDGDTTRQRIKFSDDRVCKSHLLNCCPHDVLSGTRMDLGECLKVHDLALRADYEIASKEQDFFFELDAMDHLQSFIADCDRRTEVAKKRLAETQEEISAEVAAKAERVHELNEEIGKLLAKVEQLGAEGNVEESQKVMDEVEKARAKKREAEEVYRNSMPASSFQQQKLRVCEVCSAYLGLHDNDRRLADHFGGKLHLGFIEIREKLEELKRVVAEKQEKRNQERLKRREEREREEREKLRRSRSHSKNPKRSRSREHRRHRSRSMSRERKRRTRSKSREKRHRHRSRSSSRSRSRSHQRSRHSSRDRSRERSKRRSSKERFRDQDLASCDRDRSSRDRSPRDRDRKDKKRSYESANGRSEDRRSSEEREAGEI Predict reactive species Full Name: LUC7-like 2 (S. cerevisiae) Calculated Molecular Weight: 392 aa, 47 kDa Observed Molecular Weight: 46 kDa GenBank Accession Number: BC017163 Gene Symbol: LUC7L2 Gene ID (NCBI): 51631 RRID: AB_2879455 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen Affinity purified UNIPROT ID: Q9Y383 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.