Proteintech, 24203-1-AP, ANKRD55 Polyclonal antibody

Size: 20ul / 150ul The ANKRD55 (24203-1-AP) by Proteintech is a Polyclonal antibody targeting ANKRD55 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 24203-1-AP targets ANKRD55 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, L02 cells, mouse spleen tissue, mouse small intestine tissue, mouse thymus tissue Positive IHC detected in: human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information ANKRD55 also named as Ankyrin repeat domain containing protein 55 is a 614 amino acid protein, which contains 9 ANK repeat. ANKRD55 may exist three alternatively spliced isoforms and belongs to ankyrin family. The function of ANKRD55 is still non-known and need to be further studied. ANKRD55 is a critical regulator of TCR signaling and a key regulator of T cell inflammation in multiple sclerosis (PMID: 41090353). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag21271 Product name: Recombinant human ANKRD55 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1-326 aa of BC106914 Sequence: MDSNLRDINESTPLAYALYCGHTACVKLLSQESRTEPTRPPPSQSSRPQKKERRFNVLNQIFCKNKKEEQRAHQKDPSRDRYREEDTSEVNDIITTFDSIVGTNCQEQPGDQVAMVEFKKKTSDNSKYLLPEKKPLARKGLPPIRTQSLPPITLGNNFLTASHRATSHAGLSSAPHHMAQRSQKSRSEQDLLNNRTGCQMLLDNPWKSDSNQVFSYKVWTVSSSDKLLDRLLSVRPGHQEVSVPPHLRHLHNPSSGQNFQHLSPNRHKIRDLPFTRNNLAPLPDQKFLSGEPLRTNRVLPAIPSQRRHSTAAEESEHSANPTSDEN Predict reactive species Full Name: ankyrin repeat domain 55 Calculated Molecular Weight: 614 aa, 68 kDa Observed Molecular Weight: 68 kDa GenBank Accession Number: BC106914 Gene Symbol: ANKRD55 Gene ID (NCBI): 79722 RRID: AB_2879456 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q3KP44 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.