Proteintech, 24295-1-AP, Coagulation Factor II Polyclonal antibody

Size: 20ul / 150ul The Coagulation Factor II (24295-1-AP) by Proteintech is a Polyclonal antibody targeting Coagulation Factor II in WB, IF/ICC, IP, ELISA applications with reactivity to human samples 24295-1-AP targets Coagulation Factor II in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human plasma tissue Positive IP detected in: human plasma tissue Positive IF/ICC detected in: HUVEC cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Coagulation Factor II (F2), also known as prothrombin, is one of the components of this chain of plasma proteins involved in blood coagulation. Prothrombin is the precursor of thrombin, which is essential in the processes of hemostasis and thrombosis. Specification Tested Reactivity: human Cited Reactivity: human, plasmodium falciparum Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag18385 Product name: Recombinant human F2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 273-622 aa of BC051332 Sequence: WCYVAGKPGDFGYCDLNYCEEAVEEETGDGLDEDSDRAIEGRTATSEYQTFFNPRTFGSGEADCGLRPLFEKKSLEDKTERELLESYIDGRIVEGSDAEIGMSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTENDLLVRIGKHSRTRYERNIEKISMLEKIYIHPRYNWRENLDRDIALMKLKKPVAFSDYIHPVCLPDRETAASLLQAGYKGRVTGWGNLKETWTANVGKGQPSVLQVVNLPIVERPVCKDSTRIRITDNMFCAGYKPDEGKRGDACEGDSGGPFVMKSPFNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKVIDQFGE Predict reactive species Full Name: coagulation factor II (prothrombin) Calculated Molecular Weight: 622 aa, 70 kDa Observed Molecular Weight: 72 kDa GenBank Accession Number: BC051332 Gene Symbol: F2 Gene ID (NCBI): 2147 RRID: AB_2879478 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P00734 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.