Proteintech, 24315-1-AP, BCORL1 Polyclonal antibody

Size: 20ul / 150ul The BCORL1 (24315-1-AP) by Proteintech is a Polyclonal antibody targeting BCORL1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 24315-1-AP targets BCORL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: K-562 cells, HEK-293 cells, HuH-7 cells Positive IHC detected in: mouse testis tissue, mouse colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information BCORL1 is a transcriptional corepressor found tethered to promoter regions by DNA-binding proteins. BCORL1 can interact with class II histone deacetylases, and interact with the CtBP corepressor through the CtBP binding motif-PXDLS (PMID: 21989985, 17379597). The open reading frame of BCORL1 cDNA encodes a protein of 1711 amino acids. The antibody recognized a band migrating through SDS-polyacrylamide gels at a size of ∼200 kDa. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag19522 Product name: Recombinant human BCORL1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC156742 Sequence: MISTAPLYSGVHNWTSSDRIRMCGINEERRAPLSDEESTTGDCQHFGSQEFCVSSSFSKVELTAVGSGSNARGADPDGSATEKLGHKSEDKPDDPQPKMDYAGNVAEAEGFLVPLSSPGDGLKLPASDSAEASNSRADCSWTPLNTQMSKQVDCSPAGVKALDSRQGVGEKNTFILATLGTGVPVEGTLPLVTTNFSPLPAPICPPAPGSASVPHSVPDAFQVPLSVPAPVPHSGLVPVQVATSVPAPSPPLAPVPALAPAPPSVPTLISDSNPLSVSASVLVPVPASAPPSGPVPLSAPAPAPLSVPVSAPPLALIQAPVPPSAPTLVLAPVPTPVLAPMPASTPPAA Predict reactive species Full Name: BCL6 co-repressor-like 1 Calculated Molecular Weight: 1711 aa, 183 kDa Observed Molecular Weight: 200 kDa GenBank Accession Number: BC156742 Gene Symbol: BCORL1 Gene ID (NCBI): 63035 RRID: AB_3669432 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q5H9F3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.