Proteintech, 24376-1-AP, CCDC65 Polyclonal antibody

Size: 20ul / 150ul The CCDC65 (24376-1-AP) by Proteintech is a Polyclonal antibody targeting CCDC65 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 24376-1-AP targets CCDC65 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse testis tissue, rat testis tissue Positive IP detected in: mouse testis tissue Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CCDC65, also known as DRC2, is a sperm tail protein highly expressed in adult testis, spermatocytes, and spermatids. It is a component of the nexin-dynein regulatory complex (N-DRC), a structure important for regulating and orchestrating dynein activity. Mutations in CCDC65 are associated with primary ciliary dyskinesia (PCD), a genetic disorder that affects the structure and function of cilia(PMID: 23991085). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag19357 Product name: Recombinant human CCDC65 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 12-325 aa of BC039317 Sequence: LSDEKQLLLFQQKLLAEEEMAKKKERLLSQFLKDKLAKEEHNSALNLNKINTQWRTVLREVKTRELHKDIEILSQTFERVVDCKDNVIKSLAKDLSEAEEQYAHALRSHLHNVDQLLALQRHRLSLLEESYNMELEALTKEFETERKTIIDQHEKEIHYLQDIFMAMEQNYIDSEYESKLEFQSMWNDLKNMNLEEKHFLRLHLENRVEDLWRKFQDVLKNYTDATEDRKAAFETLQVKDEKSSKEIEVQMKKIQKLQDAITISKGKIMIHSRESEDENRYIRNDKELVLVQLRKLKAQRTQARAASQKNLVRL Predict reactive species Full Name: coiled-coil domain containing 65 Calculated Molecular Weight: 484 aa, 57 kDa Observed Molecular Weight: 57 kDa GenBank Accession Number: BC039317 Gene Symbol: CCDC65 Gene ID (NCBI): 85478 RRID: AB_2879513 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8IXS2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.