Proteintech, 24403-1-AP, TRIM16 Polyclonal antibody

Size: 20ul / 150ul The TRIM16 (24403-1-AP) by Proteintech is a Polyclonal antibody targeting TRIM16 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 24403-1-AP targets TRIM16 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells Positive IP detected in: HeLa cells Positive IHC detected in: human testis tissue, human small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information TRIM16 (tripartite motif-containing protein 16) is an E3 ubiquitin ligase belonging to the tripartite motif (TRIM) protein family, mediates the ubiquitination of its target proteins and tags them for degradation. TRIM16 controls ubiquitination of damaged compartments and regulates the core autophagy regulators ULK1, Beclin 1, and ATG16L1, which confer localized autophagic sequestration of damaged lysosomes (PMID: 27693506, 22629402). TRIM16 overexpression also enhances retinoidinduced differentiation, reduces neuroblastoma cell growth, migration, and significantly reduces tumorigenicity in vivo (PMID: 36504902). Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag21406 Product name: Recombinant human TRIM16 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-349 aa of BC015674 Sequence: EMQFGELLAAVRKAQANVMLFLEEKEQAALSQANGIKAHLEYRSAEMEKSKQELERMAAISNTVQFLEEYCKFKNTEDITFPSVYVGLKDKLSGIRKVITESTVHLIQLLENYKKKLQEFSKEEEYDIRTQVSAVVQRKYWTSKPEPSTREQFLQYAYDITFDPDTAHKYLRLQEENRKVTNTTPWEHPYPDLPSRFLHWRQVLSQQSLYLHRYYFEVEIFGAGTYVGLTCKGIDRKGEERNSCISGNNFSWSLQWNGKEFTAWYSDMETPLKAGPFRRLGVYIDFPGGILSFYGVEYDTMTLVHKFACKFSEPVYAAFWLSKKENAIRIVDLGEEPEKPAPSLVGTAP Predict reactive species Full Name: tripartite motif-containing 16 Calculated Molecular Weight: 64 kDa Observed Molecular Weight: 64 kDa GenBank Accession Number: BC015674 Gene Symbol: TRIM16 Gene ID (NCBI): 10626 RRID: AB_2879525 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95361 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.