Proteintech, 24618-1-AP, RNF167 Polyclonal antibody

Size: 20ul / 150ul The RNF167 (24618-1-AP) by Proteintech is a Polyclonal antibody targeting RNF167 in WB, IF/ICC, ELISA applications with reactivity to human, mouse samples 24618-1-AP targets RNF167 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: MDA-MB-231 cells, mouse brain tissue, mouse testis tissue, SH-SY5Y cells, HeLa cells, MCF-7 cells Positive IF/ICC detected in: HaCaT cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information RNF167 also named as, ring finger protein 167 or LP2254, is a 350 amino acid protein, which contains one protease associated domain and one Ring-type zinc finger. RNF167 as single-pass membrane protein localizes in the endomembrane system and is strongly expressed in the kidney and liver. RNF167 may act as an E3 ubiquitin-protein ligase,or as a part of the E3 complex, which accepts ubiquitin from specific E3 ubiquitin from ubiquitin conjugation enzymes. RNF167 also may play a role in growth regulation in G1/S transition. The molecular weight of RNF167 is 38 kDa, but we detected a 47 kDa protein by western blot. Our result is similar to the figure of one research paper (PMID:23129617). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag18383 Product name: Recombinant human RNF167 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 26-200 aa of BC010139 Sequence: IRATSDHNASMDFADLPALFGATLSQEGLQGFLVEAHPDNACSPIAPPPPAPVNGSVFIALLRRFDCNFDLKVLNAQKAGYGAAVVHNVNSNELLNMVWNSEEIQQQIWIPSVFIGERSSEYLRALFVYEKGARVLLVPDNTFPLGYYLIPFTGIVGLLVLAMGAVMIARCIQHR Predict reactive species Full Name: ring finger protein 167 Calculated Molecular Weight: 350 aa, 38 kDa Observed Molecular Weight: 46-50 kDa GenBank Accession Number: BC010139 Gene Symbol: RNF167 Gene ID (NCBI): 26001 RRID: AB_2879642 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H6Y7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.