Proteintech, 24667-1-AP, TBC1D4 Polyclonal antibody

Size: 20ul / 150ul The TBC1D4 (24667-1-AP) by Proteintech is a Polyclonal antibody targeting TBC1D4 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, rat samples 24667-1-AP targets TBC1D4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: HCT 116 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, PC-12 cells Positive IHC detected in: mouse colon tissue, human colon tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information TBC1D4, also named as AS160, KIAA0603, may act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. The antibody recognizes all the isoform of TBC1D4. Specification Tested Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag20327 Product name: Recombinant human TBC1D4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-200 aa of BC151239 Sequence: MEPPSCIQDEPFPHPLEPEPGVSAQPGPGKPSDKRFRLWYVGGSCLDHRTTLPMLPWLMAEIRRRSQKPEAGGCGAPAAREVILVLSAPFLRCVPAPGAGASGGTSPSATQPNPAVFIFEHKAQHISRFIHNSHDLTYFAYLIKAQPDDPESQMACHVFRATDPSQVPDVISSIRQLSKAAMKEDAKPSKDNEDAFYNSQ Predict reactive species Full Name: TBC1 domain family, member 4 Calculated Molecular Weight: 1299 aa, 147 kDa Observed Molecular Weight: 160 kDa GenBank Accession Number: BC151239 Gene Symbol: TBC1D4 Gene ID (NCBI): 9882 RRID: AB_3085747 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O60343 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.