Proteintech, 24820-1-AP, C1RL Polyclonal antibody

Size: 20ul / 150ul The C1RL (24820-1-AP) by Proteintech is a Polyclonal antibody targeting C1RL in WB, IF/ICC, ELISA applications with reactivity to human, mouse samples 24820-1-AP targets C1RL in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: human plasma, HL-60 cells, K-562 cells, Ramos cells Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information C1r-like serine protease analog protein (CLSPa), also named as CIRL contains a CUB domain and a serine protease domain, possessing characteristic catalytic triad. C1RL is upregulated in glioblastoma (GBM) and has been proved to be a prognostic marker in hepatocellular carcinoma and renal cell cancer (PMID: 32993564). C1RL has a calculated molecular weight of 53 kDa, and this antibody can detect a band at 70 kDa due to possible N-glycosylation (PMID: 15358180). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag20324 Product name: Recombinant human C1RL protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 41-195 aa of BC156780 Sequence: SVLLAQELPQQLTSPGYPEPYGKGQESSTDIKAPEGFAVRLVFQDFDLEPSQDCAGDSVTISFVGSDPSQFCGQQGSPLGRPPGQREFVSSGRSLRLTFRTQPSSENKTAHLHKGFLALYQTVAVNYSQPISEASRGSEAINAPGDNPAKVQNHC Predict reactive species Full Name: complement component 1, r subcomponent-like Calculated Molecular Weight: 487 aa, 53 kDa Observed Molecular Weight: 53 kDa, 70 kDa GenBank Accession Number: BC156780 Gene Symbol: C1RL Gene ID (NCBI): 51279 RRID: AB_3085758 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NZP8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.