Proteintech, 24997-1-AP, BAALC Polyclonal antibody

Size: 20ul / 150ul The BAALC (24997-1-AP) by Proteintech is a Polyclonal antibody targeting BAALC in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 24997-1-AP targets BAALC in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, A375 cells, mouse brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Brain and acute leukemia cytoplasmic protein, also named as BAALC, is a 180 amino acid protein, which interacts with CAMK2A. BAALC localizes in the cytoplasm and membrane. BAALC is expressed in neural and hematopoietic cells. Expression is found both in normal early progenitor cells and in the most immature type of blasts in acute leukemia but not in mature hematopoietic cells. BAALC may play a synaptic role at the postsynaptic lipid rafts by interacting with CAMK2A. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag21850 Product name: Recombinant human BAALC protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-145 aa of BC011517 Sequence: MGCGGSRADAIEPRYYESWTRETESTWLTYTDSDAPPSAAAPDSGPEAGGLHSGMLEDGLPSNGVPRSTAPGGIPNPEKKTNCETQCPNPQSLSSGPLTQKQNGLQTTEAKRDAKRMPAKEVTINVTDSIQQMDRSRRITKNCVN Predict reactive species Full Name: brain and acute leukemia, cytoplasmic Calculated Molecular Weight: 19 kDa Observed Molecular Weight: 19-25 kDa GenBank Accession Number: BC011517 Gene Symbol: BAALC Gene ID (NCBI): 79870 RRID: AB_2879837 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8WXS3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.