Proteintech, 25088-1-AP, CLIP-115/CLIP2 Polyclonal antibody

Size: 20ul / 150ul The CLIP-115/CLIP2 (25088-1-AP) by Proteintech is a Polyclonal antibody targeting CLIP-115/CLIP2 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 25088-1-AP targets CLIP-115/CLIP2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: RAW 264.7 cells, A549 cells, rat brain tissue, mouse brain tissue, mouse cerebellum tissue, rat cerebellum tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:400-1:1600 Background Information CILP-2/CLIP-115 is a cytoplasmic linker protein (CLIP) that links membranous organelles to microtubules. CLIP-115 is enriched in the axonal growth cone and it enables neuronal polarization by controlling the stabilization of microtubules. Mutation of CLIP-115 has been associated with Williams-Beuren syndrome. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag18692 Product name: Recombinant human CLIP2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 671-1011 aa of BC150332 Sequence: QLEVQASQHRLELQEAQDQRRDAELRVHELEKLDVEYRGQAQAIEFLKEQISLAEKKMLDYERLQRAEAQGKQEVESLREKLLVAENRLQAVEALCSSQHTHMIESNDISEETIRTKETVEGLQDKLNKRDKEVTALTSQTEMLRAQVSALESKCKSGEKKVDALLKEKRRLEAELETVSRKTHDASGQLVLISQELLRKERSLNELRVLLLEANRHSPGPERDLSREVHKAEWRIKEQKLKDDIRGLREKLTGLDKEKSLSDQRRYSLIDPSSAPELLRLQHQLMSTEDALRDALDQAQQVEKLMEAMRSCPDKAQTIGNSGSANGIHQQDKAQKQEDKH Predict reactive species Full Name: CAP-GLY domain containing linker protein 2 Calculated Molecular Weight: 1011 aa, 112 kDa Observed Molecular Weight: 110-120 kDa GenBank Accession Number: BC150332 Gene Symbol: CLIP2 Gene ID (NCBI): 7461 RRID: AB_2879890 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UDT6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.