Proteintech, 25095-1-AP, IGFN1 Polyclonal antibody

Size: 20ul / 150ul The IGFN1 (25095-1-AP) by Proteintech is a Polyclonal antibody targeting IGFN1 in WB, ELISA applications with reactivity to human samples 25095-1-AP targets IGFN1 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A431 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Background Information Immunoglobulin-like and fibronectin type III domain-containing protein 1 (IGFN1), also known as EEF1A2-binding protein 1 (EEF1A2BP1) or KY-interacting protein 1 (KYIP1), presents structural similarity to sarcomeric proteins belonging to the intracellular Ig superfamily, including myosin-binding protein C fast and slow-type skeletal muscle isoforms (PMID: 18756455). IGFN1 along with KY and FLNC is the part of a Z-band associated protein complex likely providing structural support to the skeletal muscle (PMID: 20206623). IGFN1 gene has been associated with some cancers (PMID: 28027327; 30335789; 35456975). At least five isoforms of IGFN1 produced by alternative splicing have been identified (PMID: 20206623). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag18731 Product name: Recombinant human IGFN1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 42-385 aa of BC131529 Sequence: IYGERRDATRSSTSRYKPGTGSFSKDAQGPMGHFSQGLADMEVQPGEAATLSCTLTSDLGPGTWFKDGVKLTTQDGVIFKQDGLVHSLFITHVQGTQAGRYTFVAGDQQSEATLTVQDSPTIAPDVTEKLREPLVVKAGKPVIVKIPFQSHLPIQAAWRKDGAEVVGSSDREAQVDLGDGYTRLCLPSAGRKDCGQYSVTLRSEGGSVQAELTLQVIDKPDPPQGPMEVQDCHRAGVCLRWRPPRDNGGRTVECYVVERRQAGRSTWLKVGEAPADSTTFTDAHVEPGRKYTFRVRAVTSEGAGEALESEEILVAPEALPKAPSAPAILSASSQGITLTWTAPR Predict reactive species Full Name: immunoglobulin-like and fibronectin type III domain containing 1 Calculated Molecular Weight: 1251 aa, 138 kDa Observed Molecular Weight: 100 kDa GenBank Accession Number: BC131529 Gene Symbol: IGFN1 Gene ID (NCBI): 91156 RRID: AB_3669462 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86VF2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.