Proteintech, 25155-1-AP, ATG2B Polyclonal antibody

Size: 20ul / 150ul The ATG2B (25155-1-AP) by Proteintech is a Polyclonal antibody targeting ATG2B in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 25155-1-AP targets ATG2B in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells Positive IHC detected in: human pancreas cancer tissue, human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag18412 Product name: Recombinant human ATG2B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1304-1555 aa of BC015016 Sequence: RDYVRVMDMGLLELTITAVKSDSDGEQTEPRFELHCSSDVVHIRTCSDSCAALMNLIQYIASYGDLQTPNKADMKPGAFQRRSKVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEIDMQQGTSSVKPQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQESGPTYASFSHHFISDAMTGVPTENDDFCILFAPKAAMQEKEEEPVIKIMVDDAIVIRDNYFSLPVNKTDTSKAPLHFPIPVIRYVVKEVS Predict reactive species Full Name: ATG2 autophagy related 2 homolog B (S. cerevisiae) Calculated Molecular Weight: 2078 aa, 233 kDa Observed Molecular Weight: 240-250 kDa GenBank Accession Number: BC015016 Gene Symbol: ATG2B Gene ID (NCBI): 55102 RRID: AB_2810874 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96BY7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.