Proteintech, 25215-1-AP, ZBTB11 Polyclonal antibody

Size: 20ul / 150ul The ZBTB11 (25215-1-AP) by Proteintech is a Polyclonal antibody targeting ZBTB11 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 25215-1-AP targets ZBTB11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse liver tissue, PC-3 cells Positive IHC detected in: human urothelial carcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Zinc finger and BTB domain containing 11 (ZBTB11), also named as ZNF U69274, is a 1053 amino acid protein, which contains one BTB domain and twelve C2H2-type zinc fingers. ZBTB11 localizes in the nucleus and may be involved in transcriptional regulation. The molecular weight of ZBTB11 is 119 kDa, but the phosphorylated ZBTB11 is a 135-140 kDa protein. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag17000 Product name: Recombinant human ZBTB11 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 704-1053 aa of BC111700 Sequence: QKQFQCELCVKSFVTKRSLQEHMSIHTGESKYLCSVCGKSFHRGSGLSKHFKKHQPKPEVRGYHCTQCEKSFFEARDLRQHMNKHLGVKPFQCQFCDKCYSWKKDWYSHVKSHSVTEPYRCNICGKEFYEKALFRRHVKKATHGKKGRAKQNLERVCEKCGRKFTQLREYRRHMNNHEGVKPFECLTCGVAWADARSLKRHVRTHTGERPYVCPVCSEAYIDARTLRKHMTKFHRDYVPCKIMLEKDTLQFHNQGTQVAHAVSILTAGMQEQESSGPQELETVVVTGETMEALEAVAATEEYPSVSTLSDQSIMQVVNYVLAQQQGQKLSEVAEAIQTVKVEVAHISGGE Predict reactive species Full Name: zinc finger and BTB domain containing 11 Calculated Molecular Weight: 1053 aa, 119 kDa Observed Molecular Weight: 135 kDa GenBank Accession Number: BC111700 Gene Symbol: ZBTB11 Gene ID (NCBI): 27107 RRID: AB_2879964 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95625 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.