Proteintech, 25292-1-AP, EXOSC7 Polyclonal antibody

Size: 20ul / 150ul The EXOSC7 (25292-1-AP) by Proteintech is a Polyclonal antibody targeting EXOSC7 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 25292-1-AP targets EXOSC7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A431 cells, COLO 320 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, Caco-2 cells Positive IHC detected in: human colon tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. EXOSC7 also named as RRP42 or EAP1 is a 291 amino acid protein, which belongs to the RNase PH family. EXOSC7 is one of the six RNase-PH domain subunits of the exosome and required for the processing of the 7S pre-RNA Specification Tested Reactivity: human, mouse Cited Reactivity: human, yeast Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag18212 Product name: Recombinant human EXOSC7 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-291 aa of BC012831 Sequence: MASVTLSEAEKVYIVHGVQEDLRVDGRGCEDYRCVEVETDVVSNTSGSARVKLGHTDILVGVKAEMGTPKLEKPNEGYLEFFVDCSASATPEFEGRGGDDLGTEIANTLYRIFNNKSSVDLKTLCISPREHCWVLYVDVLLLECGGNLFDAISIAVKAALFNTRIPRVRVLEDEEGSKDIELSDDPYDCIRLSVENVPCIVTLCKIGYRHVVDATLQEEACSLASLLVSVTSKGVVTCMRKVGKGSLDPESIFEMMETGKRVGKVLHASLQSVLHKEESLGPKRQKVGFLG Predict reactive species Full Name: exosome component 7 Calculated Molecular Weight: 291 aa, 32 kDa Observed Molecular Weight: 37 kDa GenBank Accession Number: BC012831 Gene Symbol: EXOSC7 Gene ID (NCBI): 23016 RRID: AB_2880011 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q15024 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.