Proteintech, 25304-1-AP, DTX3 Polyclonal antibody

Size: 20ul / 150ul The DTX3 (25304-1-AP) by Proteintech is a Polyclonal antibody targeting DTX3 in WB, IHC, ELISA applications with reactivity to human, mouse samples 25304-1-AP targets DTX3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse kidney tissue, mouse ovary tissue, mouse testis tissue, mouse brain tissue, hTERT-RPE1 cells, rat testis tissue Positive IHC detected in: human kidney tissue, human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information DTX3, also named as Deltex3 or RING finger protein 154, is 347 amino acid protein, which contains one RING-type zinc finger and belongs to the Deltex family. DTX3 is a regulator of Notch signaling, which is involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag18111 Product name: Recombinant human DTX3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-249 aa of BC114498 Sequence: MSFVLSRMAACGGTCKNKVTVSKPVWDFLSKETPARLARLREEHRVSILIDGETSDIYVLQLSPQGPPPAPPNGLYLARKALKGLLKEAEKELKKAQRQGELMGCLALGGGGEHPEMHRAGPPPLRAAPLLPPGARGLPPPPPPLPPPLPPRLREEAEEQESTCPICLGEIQNAKTLEKCRHSFCEGCITRALQVKKACPMCGRFYGQLVGNQPQNGRMLVSKDATLLLPSYEKYGTIVIQYVFPPGVQ Predict reactive species Full Name: deltex homolog 3 (Drosophila) Calculated Molecular Weight: 347 aa, 38 kDa Observed Molecular Weight: 38 kDa GenBank Accession Number: BC114498 Gene Symbol: DTX3 Gene ID (NCBI): 196403 RRID: AB_2880021 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8N9I9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.