Proteintech, 25332-1-AP, TRH Polyclonal antibody

Size: 20ul / 150ul The TRH (25332-1-AP) by Proteintech is a Polyclonal antibody targeting TRH in WB, IHC, ELISA applications with reactivity to human samples 25332-1-AP targets TRH in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, BxPC-3 cells Positive IHC detected in: human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information TRH, Thyrotropin-releasing hormone, as a component of the hypothalamic-pituitary-thyroid axis, controls the secretion of thyroid-stimulating hormone (TSH) and is involved in thyroid hormone synthesis regulation. It also operates as modulator of hair growth and promotes hair-shaft elongation, prolongs the hair cycle growth phase (anagen) and antagonizes its termination (catagen) by TGFB2. It stimulates proliferation and inhibits apoptosis of hair matrix keratinocytes. TRH is expressed in hypothalamus and the hair follicle epithelium (PMID: 19825978). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12623 Product name: Recombinant human TRH protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-242 aa of BC074889 Sequence: MPGPWLLLALALTLNLTGVPGGRAQPEAAQQEAVTAAEHPGLDDFLRQVERLLFLRENIQRLQGDQGEHSASQIFQSDWLSKRQHPGKREEEEEEGVEEEEEEEGGAVGPHKRQHPGRREDEASWSVDVTQHKRQHPGRRSPWLAYAVPKRQHPGRRLADPKAQRSWEEEEEEEEREEDLMPEKRQHPGKRALGGPCGPQGAYGQAGLLLGLLDDLSRSQGAEEKRQHPGRRAAWVREPLEE Predict reactive species Full Name: thyrotropin-releasing hormone Calculated Molecular Weight: 242 aa, 27 kDa Observed Molecular Weight: 27-33 kDa GenBank Accession Number: BC074889 Gene Symbol: TRH Gene ID (NCBI): 7200 RRID: AB_3085786 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P20396 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.