Proteintech, 25349-1-AP, SENP1 Polyclonal antibody

Size: 20ul / 150ul The SENP1 (25349-1-AP) by Proteintech is a Polyclonal antibody targeting SENP1 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 25349-1-AP targets SENP1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HL-60 cells, HeLa cells, HepG2 cells, Jurkat cells, mouse testis tissue, rat testis tissue Positive IP detected in: Jurkat cells Positive IHC detected in: human ovary cancer tissue, human liver tissue, human ovary tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information SENP1, Sentrin-specific protease 1, is a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. SENP1 catalyzes two functions in the SUMO pathway (PMID: 29506078).The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins (PMID: 16253240). SENP1 is located in the nucleus and cytosol. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag18028 Product name: Recombinant human SENP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-331 aa of BC045639 Sequence: MDDIADRMRMDAGEVTLVNHNSVFKTHLLPQTGFPEDQLSLSDQQILSSRQGHLDRSFTCSTRSAAYNPSYYSDNPSSDSFLGSGDLRTFGQSANGQWRNSTPSSSSSLQKSRNSRSLYLETRKTSSGLSNSFAGKSNHHCHVSAYEKSFPIKPVPSPSWSGSCRRSLLSPKKTQRRHVSTAEETVQEEEREVYRQLLQMVTGKQFTIAKPTTHFPLHLSRCLSSSKNTLKDSLFKNGNSCASQIIGSDTSSSGSASILTNQEQLSHSVYSLSSYTPDVAFGSKDSGTLHHPHHHHSVPHQPDNLAASNTQSEGSDSVILLKVKDSQTPTP Predict reactive species Full Name: SUMO1/sentrin specific peptidase 1 Calculated Molecular Weight: 644 aa, 73 kDa Observed Molecular Weight: 73-74 kDa GenBank Accession Number: BC045639 Gene Symbol: SENP1 Gene ID (NCBI): 29843 RRID: AB_2880039 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9P0U3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.