Proteintech, 25465-1-AP, TWIST1-specific Polyclonal antibody

Size: 20ul / 150ul The TWIST1-specific (25465-1-AP) by Proteintech is a Polyclonal antibody targeting TWIST1-specific in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 25465-1-AP targets TWIST1-specific in WB, IHC, IF/ICC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: U-251 cells, Raji cells, A549 cells, SH-SY5Y cells, MDA-MB-231 cells, PC-3 cells Positive IHC detected in: human liver cancer tissue, human breast cancer tissue, human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: L02 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200 Background Information Twist-related protein 1 (TWIST1), also named as BHLHA38, is 202 amino acid protein, which contains one bHLH domain. TWIST1 localizes in the nucleus and acts as a transcriptional regulator. TWIST1 inhibits monogenesis by sequestrating E proteins and inhibits tans-activation by MEF2. TWIST1 is expressed in subset of mesodermal cells. The antibody reacts specifically with Twist1 protein. The calculated molecular weight of TWIST1 is 21 kDa, but the observed molecular weight is about 30 kDa due to the post-translational modification(PMID: 30733340). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat, pig, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag21924 Product name: Recombinant human TWIST1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 12-72 aa of BC036704 Sequence: PADDSLSNSEEEPDRQQPPSGKRGGRKRRSSRRSAGGGAGPGGAAGGGVGGGDEPGSPAQG Predict reactive species Full Name: twist homolog 1 (Drosophila) Calculated Molecular Weight: 202 aa, 21 kDa Observed Molecular Weight: 25-30 kDa GenBank Accession Number: BC036704 Gene Symbol: TWIST1 Gene ID (NCBI): 7291 RRID: AB_2880093 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q15672 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.