Proteintech, 25533-1-AP, KANSL3 Polyclonal antibody

Size: 20ul / 150ul The KANSL3 (25533-1-AP) by Proteintech is a Polyclonal antibody targeting KANSL3 in WB, IHC, ELISA applications with reactivity to human samples 25533-1-AP targets KANSL3 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, Jurkat cells, U2OS cells Positive IHC detected in: human intrahepatic cholangiocarcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information KANSL3, or KAT8-associated nonspecific lethal complex subunit 3, is a component of the KANSL complex, which is an important chromatin modifier in higher animals. This complex plays a crucial role in regulating housekeeping genes and is highly conserved across species. KANSL3, along with other KANSL complex members, is essential for cell division and has been shown to interact robustly both in vitro and in vivo. KANSL3 has been found to have a dual role in cellular function. In interphase, KANSL3 is chromatin-bound and regulates the expression of housekeeping genes. During mitosis, KANSL3 relocates to the mitotic spindle and is important for cell division. It has been identified as a microtubule minus-end-binding protein, which is a novel function for a chromatin-binding complex. KANSL3's ability to bind directly to microtubule minus ends is significant, as very few proteins are known to perform this function. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag22117 Product name: Recombinant human KIAA1310 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 530-878 aa of BC063792 Sequence: LSSVSSSPTSSPKTKVTTVTSAQKSSQIGSSQLLKRHVQRTEAVLTHKQAQVPISSEPPEEGEKEDLRVQLKRHHPSSPLPGSKTSKRPKIKVSLISQGDTAGGPCAPSQGSAPEAAGGKPITMTLGQASAGAKELTGLLTTAKSSSSEGGVSASPVPSVVSSSTAPSALHTLQSRLVATSPGSSLPGATSASSLLQGLSFSLQDISSKTSGLPANPSPGPAPQATSVKLPTPMQSLGAITTGTSTIVRTIPVATTLSSLGATPGGKPTAIHQLLTNGGLAKLASSLPGLAQISNQASGLKVPTTITLTLRGQPSRITTLSPMGSGAAPSEESSSQVLPSSSQRLPPAP Predict reactive species Full Name: KIAA1310 Calculated Molecular Weight: 878 aa, 93 kDa Observed Molecular Weight: 105 kDa GenBank Accession Number: BC063792 Gene Symbol: KANSL3 Gene ID (NCBI): 55683 RRID: AB_3085802 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9P2N6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.