Proteintech, 25701-1-AP, TTC28 Polyclonal antibody

Size: 20ul / 150ul The TTC28 (25701-1-AP) by Proteintech is a Polyclonal antibody targeting TTC28 in WB, IP, ELISA applications with reactivity to human samples 25701-1-AP targets TTC28 in WB, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, LNCaP cells Positive IP detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information TTC28 is a large protein required during the cell cycle for condensation of spindle midzone microtubules, formation of the midbody, and completion of cytokinesis. RT-PCR ELISA detected highest TTC28 expression in adult ovary and lung, followed by kidney and liver. Lower expression was detected in all other adult and fetal tissues examined except testis, which showed no expression. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag22465 Product name: Recombinant human TTC28 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 906-1247 aa of BC016465 Sequence: TGESDQSSTETDSTVKSQEESNPKLDPQELAQKILEETQSHLIAVERLQRSGGQVSKSNNPEDGVQAPSSTAVFRASETSAFSRPVLSHQKSQPSPVTVKPKPPARSSSLPKVSSGYSSPTTSEMSIKDSPSQHSGRPSPGCDSQTSQLDQPLFKLKYPSSPYSAHISKSPRNMSPSSGHQSPAGSAPSPALSYSSAGSARSSPADAPDIDKLKMAAIDEKVQAVHNLKMFWQSTPQHSTGPMKIFRGAPGTMTSKRDVLSLLNLSPRHNKKEEGVDKLELKELSLQQHDGAPPKAPPNGHWRTETTSLGSLPLPAGPPATAPARPLRLPSGNGYKFLSPGR Predict reactive species Full Name: tetratricopeptide repeat domain 28 Calculated Molecular Weight: 2481 aa, 271 kDa Observed Molecular Weight: 240-271 kDa GenBank Accession Number: BC016465 Gene Symbol: TTC28 Gene ID (NCBI): 23331 RRID: AB_3085817 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96AY4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.