Proteintech, 25892-1-AP, EDA Polyclonal antibody

Size: 20ul / 150ul The EDA (25892-1-AP) by Proteintech is a Polyclonal antibody targeting EDA in WB, IHC, ELISA applications with reactivity to human, mouse samples 25892-1-AP targets EDA in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse liver tissue Positive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information EDA (Ectodysplasin A) is a cytokine which is involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. It is a member of the tumor necrosis factor (TNF) superfamily, it orchestrates embryonic development of ectodermal structures including hair, teeth, nails, and sweat glands. EDA Functions as a ligand activating the DEATH-domain containing receptors EDAR and EDA2R, triggering NF-κB signaling cascades (PMID:11039935, 27144394, 34582123, 8696334). Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag23154 Product name: Recombinant human EDA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-132 aa of BC126143 Sequence: MGYPEVERRELLPAAAPRERGSQGCGCGGAPARAGEGNSCLLFLGFFGLSLALHLLTLCCYLELRSELRRERGAESRLGGSGTPGTSGTLSSLGGLDPDSPITSHLGQPSPKQQPLEPGEAALHSDSQDGHQ Predict reactive species Full Name: ectodysplasin A Observed Molecular Weight: 40 kDa GenBank Accession Number: BC126143 Gene Symbol: EDA Gene ID (NCBI): 1896 RRID: AB_2880286 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q92838 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.