Proteintech, 26155-1-AP, TGF beta 1 Polyclonal antibody

Size: 20ul / 150ul The TGF beta 1 (26155-1-AP) by Proteintech is a Polyclonal antibody targeting TGF beta 1 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to mouse, rat samples 26155-1-AP targets TGF beta 1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with mouse, rat samples. Tested Applications Positive WB detected in: L-929 cells, NIH/3T3 cells, RAW 264.7 cells, C6 Positive IF/ICC detected in: NIH/3T3 cells Positive FC (Intra) detected in: NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information TGF beta 1 is a potent cytokine capable of regulating multiple cellular processes including cell proliferation, differentiation, wound healing, and immune response. TGF beta 1 is identified in various immune cells and its activity varies in different cell types or cells at different developmental stages1. TGF beta 1 functions by binding to the TGFβ1 receptors, which subsequently activate the canonical SMAD pathway or non-canonical signaling cascades, such as the mitogen-activated protein kinase (MAPK) pathway, phosphatidylinositol-3 kinase and AKT (PI3K-AKT), and Rho family of GTPases (Rho GTPase). What is special about TGF beta 1 is its context-dependent nature that is particularly true for tumors. Full-length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB (12, 25, 45 to 65 kDa). Specification Tested Reactivity: mouse, rat Cited Reactivity: human, mouse, rat, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag24078 Product name: Recombinant mouse Tgfb1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 29-390 aa of NM-011577 Sequence: GLSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESADPEPEPEADYYAKEVTRVLMVDRNNAIYEKTKDISHSIYMFFNTSDIREAVPEPPLLSRAELRLQRLKSSVEQHVELYQKYSNNSWRYLGNRLLTPTDTPEWLSFDVTGVVRQWLNQGDGIQGFRFSAHCSCDSKDNKLHVEINGISPKRRGDLGTIHDMNRPFLLLMATPLERAQHLHSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASASPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS Predict reactive species Full Name: transforming growth factor, beta 1 Observed Molecular Weight: 44 kDa GenBank Accession Number: NM-011577 Gene Symbol: TGF beta 1 Gene ID (NCBI): 21803 RRID: AB_3085844 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P04202 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.