Proteintech, 26320-1-AP, C13orf3 Polyclonal antibody

Size: 20ul / 150ul The C13orf3 (26320-1-AP) by Proteintech is a Polyclonal antibody targeting C13orf3 in WB, ELISA applications with reactivity to human samples 26320-1-AP targets C13orf3 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293T cells, HL-60 cells, Jurkat cells, K-562 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information C13orf3 (now known as Ska3) is a component of a spindle- and mitophore-associated protein complex that regulates microtubule attachment to the mitophore during mitosis. Ska3 is localized to the outer side of the mitophore during mitosis and may be essential for normal chromosome segregation. In addition, deletion of Ska3 results in a delay in mid-mitotic division and an inability to silence the spindle checkpoint or enter prophase. Accumulation of Ska3 is dependent on Sgo1 proteins, whereas mitotic accumulation of Sgo1 in prophase is not dependent on Ska3. The discovery of Ska3 further refines the function of the Ska complex, which plays an important role in mitotic progression and chromosome segregation. The discovery of Ska3 in the prophase of Sgo1 is a key factor in mitosis and chromosome segregation. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag23684 Product name: Recombinant human C13orf3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-356 aa of BC048988 Sequence: MDPIRSFCGKLRSLASTLDCETARLQRALDGEESDFEDYPMRILYDLHSEVQTLKDDINILLDKARLENQEGIDFIKATKVLMEKNSMDIMKIREYFQKYGYSPRVKKNSVHEQEAINSDPELSNCENFQKTDVKDDLSDPPVASSCISEKSPRSPQLSDFGLERYIVSQVLPNPPQAVNNYKEEPVIVTPPTKQSLVKVLKTPKCALKMDDFECVTPKLEHFGISEYTMCLNEDYTMGLKNARNNKSEEAIDTESRLNDNVFATPSPIIQQLEKSDAEYTNSPLVPTFCTPGLKIPSTKNSIALVSTNYPLSKTNSSSNDLEVEDRTSLVLNSDTCFENLTDPSSPTISSYENLL Predict reactive species Full Name: chromosome 13 open reading frame 3 Observed Molecular Weight: 54 kDa GenBank Accession Number: BC048988 Gene Symbol: C13orf3 Gene ID (NCBI): 221150 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8IX90 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.