Proteintech, 26479-1-AP, Plakophilin 2 Polyclonal antibody

Size: 20ul / 150ul The Plakophilin 2 (26479-1-AP) by Proteintech is a Polyclonal antibody targeting Plakophilin 2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, rat samples 26479-1-AP targets Plakophilin 2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: Caco-2 cells, HepG2 cells, rat heart tissue Positive IP detected in: HepG2 cells Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Caco-2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Plakophilin 2 also known as PKP2, belongs to the beta-catenin family. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton (PMID: 10828611). Mutations in PKP2 are associated with Arrhythmogenic right ventricular dysplasia, familial, 9 (ARVD9)(PMID: 15489853). Specification Tested Reactivity: human, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag24155 Product name: Recombinant human PKP2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC094762 Sequence: MAAPGAPAEYGYIRTVLGQQILGQLDSSSLALPSEAKLKLAGSSGRGGQTVKSLRIQEQVQQTLARKGRSSVGNGNLHRTSSVPEYVYNLHLVENDFVGGRSPVPKTYDMLKAGTTATYEGRWGRGTAQYSSQKSVEERSLRHPLRRLEISPDSSPERAHYTHSDYQYSQRSQAGHTLHHQESRRAALLVPPRYARSEIVGVSRAGTTSRQRHFDTYHRQYQHGSVSDTVFDSIPANPALLTYPRPGTSRSMGNLLEKENYLTAGLTVGQVRPLVPLQPVTQNRASRSSWHQSSFHSTRTLREAGPSVAVDSSGRRAHLTVGQAAAGGSGNLLTERSTFTDSQLGNADMEM Predict reactive species Full Name: plakophilin 2 Calculated Molecular Weight: 837 aa, 93 kDa Observed Molecular Weight: 90-100 kDa GenBank Accession Number: BC094762 Gene Symbol: Plakophilin 2 Gene ID (NCBI): 5318 RRID: AB_3669539 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q99959 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.