Proteintech, 26833-1-AP, SLC22A16 Polyclonal antibody

Size: 20ul / 150ul The SLC22A16 (26833-1-AP) by Proteintech is a Polyclonal antibody targeting SLC22A16 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 26833-1-AP targets SLC22A16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MCF-7 cells, PC-3 cells Positive IHC detected in: human breast cancer tissue, human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: THP-1 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information SLC22A16, also named as CT2, FLIPT2, or OCT6, belongs to the major facilitator (TC 2.A.1) superfamily and organic cation transporter (TC 2.A.1.19) family. SLC22A16 encodes an organic zwitterion transporter protein that transports carnitine and some anticancer drugs, including anthracyclines. As a carnitine transporter, SLC22A16 is involved in the regulation of fatty acid oxidation (FAO), which plays an important role also in the metabolic transformation of cancer cells. SLC22A16 expression in cancer cells is associated with increased sensitivity to the cytotoxic effects of doxorubicin. SLC22A16 has 3 isoforms with the molecular mass of 58, 61 and 65 kDa (PMID: 35761855, 35608746). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag25270 Product name: Recombinant human SLC22A16 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 48-150 aa of BC047565 Sequence: PHHVCRPPGNVSQVVFHNHSNWSLEDTGALLSSGQKDYVTVQLQNGEIWELSRCSRNKRENTSSLGYEYTGSKKEFPCVDGYIYDQNTWKSTAVTQWNLVCDR Predict reactive species Full Name: solute carrier family 22 (organic cation/carnitine transporter), member 16 Calculated Molecular Weight: 65 kDa Observed Molecular Weight: 68 kDa GenBank Accession Number: BC047565 Gene Symbol: SLC22A16 Gene ID (NCBI): 85413 RRID: AB_2923576 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86VW1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.