Proteintech, 27093-1-AP, Angiopoietin 1 Polyclonal antibody

Size: 20ul / 150ul The Angiopoietin 1 (27093-1-AP) by Proteintech is a Polyclonal antibody targeting Angiopoietin 1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 27093-1-AP targets Angiopoietin 1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HUVEC cells, HeLa cells, U-87 MG cells, mouse heart tissue, rat heart tissue, U-97 MG cells Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HUVEC cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag25786 Product name: Recombinant human ANGPT1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 16-67 aa of BC152411 Sequence: HIGCSNQRRSPENSGRRYNRIQHGQCAYTFILPEHDGNCRESTTDQYNTNAL Predict reactive species Full Name: angiopoietin 1 Calculated Molecular Weight: 498 aa, 58 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC152411 Gene Symbol: Angiopoietin 1 Gene ID (NCBI): 284 RRID: AB_3085925 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q15389 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.