Proteintech, 27128-1-AP, VPS37C Polyclonal antibody

Size: 20ul / 150ul The VPS37C (27128-1-AP) by Proteintech is a Polyclonal antibody targeting VPS37C in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 27128-1-AP targets VPS37C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HT-29 cells, SK-BR-3 cells, T-47D cells Positive IHC detected in: mouse colon tissue, mouse lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Raji cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information VPS37C (Vacuolar protein sorting-associated protein 37C) is also named as hVps37C, PML39 and ESCRT-I complex subunit VPS37C. VPS37C is a core subunit of the Endosomal sorting complex required for transport-I (ESCRT-I) complex (PMID: 37307706). VPS37C interacts with EKLF and prevents K48-linked polyubiquitination of EKLF and proteasome-mediated EKLF degradation, thus enhancing EKLF protein stability and transcriptional activity (PMID: 37307706). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag26024 Product name: Recombinant human VPS37C protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-117 aa of BC141827 Sequence: METLKDKTLQELEELQNDSEAIDQLALESPEVQDLQLEREMALATNRSLAERNLEFQGPLEISRSNLSDRYQELRKLVERCQEQKAKLEKFSSALQPGTLLDLLQVEGMKIEEESEA Predict reactive species Full Name: vacuolar protein sorting 37 homolog C (S. cerevisiae) Observed Molecular Weight: 39-45 kDa GenBank Accession Number: BC141827 Gene Symbol: VPS37C Gene ID (NCBI): 55048 RRID: AB_3085928 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: A5D8V6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.