Proteintech, 27132-1-AP, Nesprin 3 Polyclonal antibody

Size: 20ul / 150ul The Nesprin 3 (27132-1-AP) by Proteintech is a Polyclonal antibody targeting Nesprin 3 in WB, ELISA applications with reactivity to human, mouse, rat samples 27132-1-AP targets Nesprin 3 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: C2C12 cells, rat spleen tissue, mouse spleen tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information Nesprin 3 also known as C14orf49, SYNE3, belongs to the Nesprin family. Nesprin-3 is a type II transmembrane protein of the outer nuclear membrane and contains a C-terminal KASH domain and a series of spectrin repeats(PMID: 16330710). Nesprin-3, a protein that links intermediate filaments to the nucleus, regulates endothelial cell morphology, perinuclear cytoskeletal organization, and flow-induced cell polarization and migration. Nesprin-3 is robustly expressed in human aortic endothelial cells and localizes to the nuclear envelope(PMID: 21937718). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag26002 Product name: Recombinant human C14orf49 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC146604 Sequence: MTQQPQDDFDRSVEDAQAWMKAVQDQLQVNDNTQGPRAALEARLWETEKICQLEPEGRVRVDLVLRMAEALLACCPGDQKPGILARLKDIKAQWEETVTYMTHCHSRIEWVWLHWSEYLLARDEFYRWFQKMMVTLEPHIELQLGLKEKQWQLSHAQVLLHNVDNQAVLLDRLLEEAASLFNRIGDPSVDEDAQKRMKAEYDAVKAKAQKRVDLLEQVAREHEEYQAGVDEFQLWLKAVVEKVNGCLGRNCKLPITQRLSTLQDIAKDFPRGEESLETLEEQSAGVIRNTSPLGAEKITG Predict reactive species Full Name: chromosome 14 open reading frame 49 Observed Molecular Weight: 100-110 kDa GenBank Accession Number: BC146604 Gene Symbol: Nesprin 3 Gene ID (NCBI): 161176 RRID: AB_2880767 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6ZMZ3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.