Proteintech, 27193-1-AP, Neuropilin 2 Polyclonal antibody

Size: 20ul / 150ul The Neuropilin 2 (27193-1-AP) by Proteintech is a Polyclonal antibody targeting Neuropilin 2 in WB, IHC, IP, ELISA applications with reactivity to human samples 27193-1-AP targets Neuropilin 2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: U-87 MG cells Positive IP detected in: U-87 MG cells Positive IHC detected in: human intrahepatic cholangiocarcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Neuropilin 2 (NRP2) is a single-chain transmembrane glycoprotein that acts as a co-receptor of VEGF. NRP2 is mainly expressed in lymphatic and venous endothelial cells, and it is associated with the occurrence and development of several types of cancer. NRP2 is known to play a role in cardiovascular development, axon guidance, tumorigenesis, inflammation and cardiovascular disease. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag25622 Product name: Recombinant human NRP2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 566-858 aa of BC104770 Sequence: IPAQYVRVYPERWSPAGIGMRLEVLGCDWTDSKPTVETLGPTVKSEETTTPYPTEEEATECGENCSFEDDKDLQLPSGFNCNFDFLEEPCGWMYDHAKWLRTTWASSSSPNDRTFPDDRNFLRLQSDSQREGQYARLISPPVHLPRSPVCMEFQYQATGGRGVALQVVREASQESKLLWVIREDQGGEWKHGRIILPSYDMEYQIVFEGVIGKGRSGEIAIDDIRISTDVPLENCMEPISAFAGGTLLPGTEPTVDTVPMQPIPAYWYYVMAAGGAVLVLVSVALALVLHYHR Predict reactive species Full Name: neuropilin 2 Observed Molecular Weight: 120-130 kDa GenBank Accession Number: BC104770 Gene Symbol: Neuropilin 2 Gene ID (NCBI): 8828 RRID: AB_3669588 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O60462 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.