Proteintech, 27311-1-AP, SERCA2/ATP2A2 Polyclonal antibody

Size: 20ul / 150ul The SERCA2/ATP2A2 (27311-1-AP) by Proteintech is a Polyclonal antibody targeting SERCA2/ATP2A2 in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications with reactivity to human, mouse samples 27311-1-AP targets SERCA2/ATP2A2 in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse heart tissue, HEK-293 cells, mouse heart unboiled, 37°C incubated HEK-293 cells Positive IHC detected in: mouse skeletal muscle tissue, mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse heart tissue Positive IF-Fro detected in: mouse heart tissue Positive IF/ICC detected in: C2C12 cells, mouse heart tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. ATP2A2 is mainly expressed in the sarcoplasmic reticulum of muscle cells and the endoplasmic reticulum of most cell types. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag26177 Product name: Recombinant human SERCA2,ATP2A2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 314-580 aa of BC035588 Sequence: VITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFILDRVEGDTCSLNEFTITGSTYAPIGEVHKDDKPVNCHQYDGLVELATICALCNDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTELKGLSKIERANACNSVIKQLMKKEFTLEFSRDRKSMSVYCTPNKPSRTSMSKMFVKGAPEGVIDRCTHIRVGSTKVPMTSGVKQKIMSVIREWGSGSDTLRCLALATHDNPLRREEMHLEDS Predict reactive species Full Name: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 Calculated Molecular Weight: 1042 aa, 115 kDa Observed Molecular Weight: 110-115 kDa GenBank Accession Number: BC035588 Gene Symbol: SERCA2 Gene ID (NCBI): 488 RRID: AB_3085945 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P16615 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.