Proteintech, 27665-1-AP, EIF3A Polyclonal antibody

Size: 20ul / 150ul The EIF3A (27665-1-AP) by Proteintech is a Polyclonal antibody targeting EIF3A in WB, IHC, IF/ICC, ELISA applications with reactivity to Human, mouse samples 27665-1-AP targets EIF3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples. Tested Applications Positive WB detected in: BxPC-3 cells, SKOV-3 cells, mouse brain tissue Positive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Specification Tested Reactivity: Human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag24752 Product name: Recombinant human EIF3A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 266-437 aa of BC114429 Sequence: PPKPQLMANYYNKVSTVFWKSGNALFHASTLHRLYHLSREMRKNLTQDEMQRMSTRVLLATLSIPITPERTDIARLLDMDGIIVEKQRRLATLLGLQAPPTRIGLINDMVRFNVLQYVVPEVKDLYNWLEVEFNPLKLCERVTKVLNWVREQPEKEPELQQYVPQLQNNTIL Predict reactive species Full Name: eukaryotic translation initiation factor 3, subunit A Calculated Molecular Weight: 1382 aa, 166 kDa Observed Molecular Weight: 166 kDa GenBank Accession Number: BC114429 Gene Symbol: EIF3A Gene ID (NCBI): 8661 RRID: AB_2880942 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q14152 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.