Proteintech, 27716-1-AP, Perilipin 1 Polyclonal antibody

Size: 20ul / 150ul The Perilipin 1 (27716-1-AP) by Proteintech is a Polyclonal antibody targeting Perilipin 1 in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse samples 27716-1-AP targets Perilipin 1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: 3T3-L1 cells, MCF-7 cells, NIH/3T3 cells, mouse liver tissue Positive IHC detected in: mouse brown adipose tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse brown adipose tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information PLIN1, also known as Perilipin 1, PLIN, PERI, belongs to the perilipin family. PLIN1 is a hormonally-regulated phosphoprotein that encircles the lipid storage droplet in adipocytes and modulates adipocyte lipid metabolism (PMID: 2040638). PLIN1 is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in inhibiting lipolysis. PLIN1 is detected in adipocytes of white adipose tissue, visceral adipose tissue, and mammary gland (PMID: 27832861, 9521880). The deficiency of PLIN1 causes lipodystrophic syndromes, which disables the optimal accumulation of triglycerides in adipocytes that results in abnormal deposition of lipids in tissues such as skeletal muscle and liver. The storage of lipids in the liver leads to insulin resistance and hypertriglyceridemia (PMID: 21345103). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, goat, sheep Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag26426 Product name: Recombinant human PLIN1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 91-200 aa of NM_001145311 Sequence: MACRGLDHLEEKIPALQYPPEKIASELKDTISTRLRSARNSISVPIASTSDKVLGAALAGCELAWGVARDTAEFAANTRAGRLASGGADLALGSIEKVVEYLLPPDKEESA Predict reactive species Full Name: perilipin Calculated Molecular Weight: 56 kDa Observed Molecular Weight: 62-67 kDa GenBank Accession Number: NM_001145311 Gene Symbol: Perilipin 1 Gene ID (NCBI): 5346 RRID: AB_3085989 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O60240 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.