Proteintech, 27785-1-AP, CSDA Polyclonal antibody

Size: 20ul / 150ul The CSDA (27785-1-AP) by Proteintech is a Polyclonal antibody targeting CSDA in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 27785-1-AP targets CSDA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Y79 cells, KYSE-30 cells, mouse liver tissue, rat liver tissue Positive IHC detected in: mouse heart tissue, mouse skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CSDA (Cold shock domain protein A), also known as DBPA (DNA-binding protein A), YBX3 (Y-box protein 3), is a DNA-binding protein that represses angiogenesis and lymphangiogenesis by directly binding to hypoxia response element (HRE) and serum response element (SRE). CSDA is ubiquitous expressed in human skeletal muscle, heart, and decidual cells, which is thought to a multifunctional epithelial-specific protein involved in gene transcription, DNA repair, and interaction with other proteins (PMID: 33816248). CSDA is a nucleic-acid-binding protein that directly regulates gene expression by using different mechanisms, and acts as a translational repressor (e.g., MYH9 and SPTBN1) and positive regulator of mRNA stability (PMID: 31189097). Western blot analysis detected CDSA at a range of 50~70 kDa due to the anomalous mobility of Y-box proteins (PMID: 38255791). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag26839 Product name: Recombinant human CSDA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC008801 Sequence: MSEAGEATTTTTTTLPQAPTEAAAAAPQDPAPKSPVGSGAPQAAAPAPAAHVAGNPGGDAAPAATGTAAAASLAAAAGSEDAEKKVLATKVLGTVKWFNVRNGYGFINRNDTKEDVFVHQTAIKKNNPRKYLRSVGDGETVEFDVVEGEKGAEAANVTGPDGVPVEGSRYAADRRRYRRGYYGRRRGPPRNYAGEEEEEGSGSSEGFDPPATDRQFSGARNQLRRPQYRPQYRQRRFPPYHVGQTFDRRSRVLPHPNRIQAGEIGEMKDGVPEGAQLQGPVHRNPTYRPRYRSRGPPRPR Predict reactive species Full Name: cold shock domain protein A Calculated Molecular Weight: 40 kDa Observed Molecular Weight: 60-68 kDa GenBank Accession Number: BC008801 Gene Symbol: CSDA Gene ID (NCBI): 8531 RRID: AB_3085996 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P16989 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.