Proteintech, 28030-1-AP, ATP1A3 (middle) Polyclonal antibody

Size: 20ul / 150ul The ATP1A3 (middle) (28030-1-AP) by Proteintech is a Polyclonal antibody targeting ATP1A3 (middle) in WB, IHC, IF/ICC, ELISA applications with reactivity to Human samples 28030-1-AP targets ATP1A3 (middle) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: HeLa cells, SH-SY5Y cells, SKOV-3 cells, U-87 MG cells Positive IHC detected in: human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information ATP1A3 participates in the catalyticing hydrolysis of ATP and the exchanging of sodium and potassium ions across plasma membrane. The catalyticing activity mode is ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In). It has been published that the neurologic disorders rapid-onset dystonia-parkionsonism (RDP), alternating hemiplegia of childhood (ACH) and CAPOS syndrome (cerebellar ataxia, areflexia, pes cavus, optic atrophy and sensorineural hearing loss) are all related with the mutation of ATP1A3. There are other reports suggest that early life epilepsy and episodic apnea revealing are potentially associated with the mutation of ATP1A3 as a result of impairment of Na+/K+homeostasis. This antibody is generated agaist the middle region (378-580aa) of ATP1A3 and detects 113 kDa protein in SDS-PAGE.(PMID: 30097153, 20301294, 29922587) Specification Tested Reactivity: Human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag27705 Product name: Recombinant human ATP1A3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 378-580 aa of BC015566 Sequence: VAHMWFDNQIHEADTTEDQSGTSFDKSSHTWVALSHIAGLCNRAVFKGGQDNIPVLKRDVAGDASESALLKCIELSSGSVKLMRERNKKVAEIPFNSTNKYQLSIHETEDPNDNRYLLVMKGAPERILDRCSTILLQGKEQPLDEEMKEAFQNAYLELGGLGERVLGFCHYYLPEEQFPKGFAFDCDDVNFTTDNLCFVGLMS Predict reactive species Full Name: ATPase, Na+/K+ transporting, alpha 3 polypeptide Calculated Molecular Weight: 113 kDa Observed Molecular Weight: 113 kDa GenBank Accession Number: BC015566 Gene Symbol: ATP1A3 Gene ID (NCBI): 478 RRID: AB_2881041 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P13637 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.