Proteintech, 28112-1-AP, L3MBTL3 Polyclonal antibody

Size: 20ul / 150ul The L3MBTL3 (28112-1-AP) by Proteintech is a Polyclonal antibody targeting L3MBTL3 in WB, IHC, IF/ICC, ELISA applications with reactivity to Human, Mouse samples 28112-1-AP targets L3MBTL3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples. Tested Applications Positive WB detected in: HEK-293T cells, HeLa cells, Jurkat cells Positive IHC detected in: mouse cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information L3MBTL3, also named as MBT1 and KIAA1798, belongs to the Malignant brain tumor (MBT) family. L3MBTL3 can bind to methyl lysine in histone, further affecting the transcription and its abnormal regulation associated with many diseases (PMID: 34373992). Additionally, researches have shown that L3MBTL3 plays a role in meduloblastoma formation and normal hematopoiesis in humans (PMID: 26317848). L3MBTL3 has 2 isoforms with the molecular mass of 86 and 88 kDa. Specification Tested Reactivity: Human, Mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag27136 Product name: Recombinant human L3MBTL3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 524-646 aa of BC060845 Sequence: LMEASEHGGCSTPGCKGIGHFKRARHLGPHSAANCPYSEINLNKDRIFPDRLSGEMPPASPSFPRNKRTDANESSSSPEIRDQHADDVKEDFEERTESEMRTSHEARGAREEPTVQQAQRRSA Predict reactive species Full Name: l(3)mbt-like 3 (Drosophila) Calculated Molecular Weight: 88 aa, 3 kDa Observed Molecular Weight: 88 kDa GenBank Accession Number: BC060845 Gene Symbol: L3MBTL3 Gene ID (NCBI): 84456 RRID: AB_2918148 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96JM7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.