Proteintech, 28156-1-AP, WNT3 Polyclonal antibody

Size: 20ul / 150ul The WNT3 (28156-1-AP) by Proteintech is a Polyclonal antibody targeting WNT3 in WB, IHC, ELISA applications with reactivity to Human, mouse samples 28156-1-AP targets WNT3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples. Tested Applications Positive WB detected in: HCT 116 cells, MCF-7 cells Positive IHC detected in: mouse colon tissue, mouse kidney tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information WNT3 is also named as INT4. WNT3 is required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis (PMID: 14872406). Wnt3 is up-regulated in cancer tissues, such as gastric cancer and lung cancer. Wnt3, as a member of Wnt family, has been proved to promote the stabilization of β-catenin to regulate the radiation sensitivity of cancer cells (PMID: 31851619). Specification Tested Reactivity: Human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag27965 Product name: Recombinant human WNT3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 130-306 aa of BC114219 Sequence: SCAEGTSTICGCDSHHKGPPGEGWKWGGCSEDADFGVLVSREFADARENRPDARSAMNKHNNEAGRTTILDHMHLKCKCHGLSGSCEVKTCWWAQPDFRAIGDFLKDKYDSASEMVVEKHRESRGWVETLRAKYSLFKPPTERDLVYYENSPNFCEPNPETGSFGTRDRTCNVTSHG Predict reactive species Full Name: wingless-type MMTV integration site family, member 3 Calculated Molecular Weight: 355 aa, 40 kDa Observed Molecular Weight: 40-45 kDa GenBank Accession Number: BC114219 Gene Symbol: WNT3 Gene ID (NCBI): 7473 RRID: AB_3669649 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P56703 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.