Proteintech, 28263-1-AP, TMEM192 Polyclonal antibody

Size: 20ul / 150ul TMEM192 Polyclonal Antibody for WB, IHC, ELISA 28263-1-AP targets TMEM192 in WB, IHC, IP, ELISA applications and shows reactivity with Human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, U2OS cells Positive IHC detected in: human breast cancer tissue, human liver cancer tissue, human lung cancer tissue, human prostate cancer tissue, mouse cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Transmembrane protein 192 (TMEM192) has been previously identified in proteomic analyses of lysosomal membranes.TMEM192 shows a wide expression pattern in mouse tissues. Interestingly, TMEM192 was found to be highly expressed in tumor cell lines, while it was not expressed or detected at low levels in normal cell lines(PMID: 22736246). TMEM192 can be detected as 30 kDa. Specification Tested Reactivity: Human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag27576 Product name: Recombinant human TMEM192 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 193-271 aa of BC036301 Sequence: KIRRFNKAKPEPDILEEEKIYAYPSNITSETGFRTISSLEEIVEKQGDTIEYLKRHNALLSKRLLALTSSDLGCQPSRT Predict reactive species Full Name: transmembrane protein 192 Observed Molecular Weight: 30 kDa GenBank Accession Number: BC036301 Gene Symbol: TMEM192 Gene ID (NCBI): 201931 RRID: AB_2881099 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8IY95 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.